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Effect of C‐Reactive Protein on Peritoneal Macrophages
Author(s) -
Miyagawa Nobuyuki,
Okamoto Yasuyuki,
Nakano Hiroshi
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01433.x
Subject(s) - superoxide , incubation , macrophage , c reactive protein , superoxide dismutase , biology , inflammation , reactive oxygen species , macrophage migration inhibitory factor , medicine , in vitro , endocrinology , immunology , biochemistry , cytokine , oxidative stress , enzyme
The effect of human C‐reactive protein (CRP) on macrophage function was studied in an assay of superoxide anion (O 2 − ) production. Peritoneal exudate macrophages (PEM) of guinea pigs exposed in vitro to various doses of CRP for 72 hr resulted in the development of O 2 − production dose‐dependently, measured by increases in superoxide dismutase‐inhibitable nitro blue tetrazolium reduction. The O 2 − ‐producing activity of PEM cultured without CRP, used as a control, decreased markedly in proportion to incubation time. The O 2 − production by PEM exposed to CRP for 18 hr when control PEM were still high in O 2 − production, was decreased by larger doses of CRP, while PEM cultured without CRP for 72 hr, when O 2 − production by control PEM was very low, followed by incubation with CRP for another 18 hr, produced O 2 − CRP‐dose‐dependently as in the case of that observed after 72‐hr incubation with CRP. These results indicate that CRP is capable of activating macrophages and acts on macrophage function as a modulator. CRP possesses migration inhibitory factor (MIF)‐like activity (as reported in the preceding paper) and also macrophage‐activating factor (MAF)‐like activity, indicating that CRP may play a functional role at the site of inflammation and tissue damage by accumulating and activating macrophages.