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Linkage of K99 Production and STa Activity in a Plasmid of an Escherichia coli Porcine Isolate
Author(s) -
Shimizu Mikio,
Sakano Tetsuya,
Satoh Shizuo
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01424.x
Subject(s) - plasmid , escherichia coli , biology , recombinant dna , microbiology and biotechnology , enterobacteriaceae , restriction enzyme , heat stable enterotoxin , genetics , gene
An Escherichia coli strain ZP118 of porcine origin was shown to harbor a 68‐megadalton (Md) plasmid coding for a colonization factor K99 and heat‐stable enterotoxin (STa), and a self‐transmissible 51‐Md plasmid coding for drug resistance. One of the transconjugants obtained by mating between ZP118 and E. coli C was found to harbor a 90‐Md plasmid coding for K99, STa and drug resistance. Restriction endonuclease analysis suggested that the 90‐Md plasmid could be a recombinant between the 68‐Md plasmid and the 51‐Md plasmid.