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Priming Activity for Chemiluminescence Reaction of PMN in the Culture Supernatant of Streptococcal Preparation (OK‐432)‐Stimulated Spleen Cells
Author(s) -
Fukase Yoko,
Fukase Shigeru,
Sendo Fujiro
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01423.x
Subject(s) - chemiluminescence , sodium azide , priming (agriculture) , catalase , spleen , respiratory burst , microbiology and biotechnology , neutrophile , taurine , biology , biochemistry , chemistry , immunology , enzyme , chromatography , in vitro , botany , germination , amino acid
We investigated the effect of supernatant from human spleen cell culture stimulated with a streptococcal preparation, OK‐432 (OK sup), on the luminol‐dependent chemiluminescence (CL) of human polymorphonuclear leukocytes (PMN). The fMLP‐stimulated CL of PMN was markedly enhanced by the pretreatment with OK sup. This result indicates that OK sup contained the factor(s) that primes fMLP‐stimulated CL of PMN. The priming factor(s) in OK sup was partially inactivated by the treatment at 56 C for 30 min and pH 2 or pH 10 treatments. Since the enhancing effect of OK sup on the CL was inhibited by the treatment of sodium azide and the addition of catalase or taurine, it was assumed that OK sup augments the activity of MPO‐H 2 O 2 ‐HOCl system of fMLP‐stimulated PMN.