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Luminol‐Dependent Chemiluminescence in Antibody‐Sensitized Neutrophils Stimulated with Protein A‐Bearing Staphylococci
Author(s) -
Nishihara Shoko,
Seki Keiko,
Ikigai Hajime,
Masuda Shogo
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01414.x
Subject(s) - staphylococcus aureus , opsonin , antibody , luminol , phagocytosis , chemiluminescence , stimulation , microbiology and biotechnology , biology , immunology , chemistry , bacteria , endocrinology , genetics , organic chemistry
When mouse polymorphonuclear leukocytes (PMNs) sensitized with rabbit antibody to mouse Ehrlich ascites tumor cells were stimulated by Staphylococcus aureus Cowan I cells, a conspicuous luminol‐dependent chemiluminescence was observed in the absence of opsonin. The profile of the chemiluminescence (CL) response evoked by staphylococcal cells from antibody‐sensitized PMNs had two peaks. An initial peak, observed within 1 min after stimulation, was sharp and high and a second peak, observed about 5 min after stimulation, was low and extended. The CL response of antibody‐sensitized PMNs stimulated by S. aureus Cowan I cells was dose‐dependently blocked by preincubation with soluble SpA. Cells of a mutant derived from S. aureus Cowan I strain with trace amounts of cell‐bound SpA failed to stimulate the antibody‐sensitized PMNs to generate the CL response. The antibody‐sensitized PMNs were found to phagocytize SpA‐bearing S. aureus cells even in the absence of opsonic serum. These results suggest that the observation presented here might provide a useful tool for the investigation of CL response of PMNs.