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Immunological Detection of 22K Protein in Sporulating Cells of Bacillus megaterium ATCC 12872
Author(s) -
Nishihara Tsutomu,
Sakagami Kimie,
Tani Katsuji,
Imagawa Masayoshi,
Kondo Masaomi
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01413.x
Subject(s) - bacillus megaterium , biology , spore , antibody , cytoplasm , microbiology and biotechnology , immunoassay , coat , biochemistry , bacteria , immunology , paleontology , genetics
The synthesis and deposition of 22,000‐dalton (22K) spore coat protein were examined immunochemically on the sporulating cells of Bacillus megaterium ATCC 12872 using the antibody to purified 22K spore coat protein. This antibody cross‐reacted with 44K and 25K proteins in immunoblot analysis of dormant spore coat proteins. Immunoblot analysis on the sporulating cells showed that 22K protein was detected from t 8 in forespore coat protein fractions. Sandwich enzyme immunoassay revealed that 22K protein in the spore coat protein fraction appeared at t 6 and reached a plateau at t 9 , and 22K protein in the mother cell cytoplasmic fraction was detected at only t 7 and t 8 at a very low level.