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Formation of a Hexagonal Lattice Structure by an R‐Form Lipopolysaccharide of Klebsiella : Effect of Various Divalent Cations on the Lattice Formation
Author(s) -
Kato Nobuo,
Ohta Michio,
Kido Nobuo,
Ito Hideo,
Naito Setsuko
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01408.x
Subject(s) - divalent , lattice constant , crystallography , crystal structure , hexagonal lattice , chemistry , lattice (music) , tris , inorganic chemistry , physics , organic chemistry , condensed matter physics , antiferromagnetism , diffraction , biochemistry , acoustics , optics
The R‐form lipopolysaccharide from Klebsiella pneumoniae strain LEN‐111 (O3‐: Kl‐), from which cationic material had been removed by electrodialysis, was previously shown to form a hexagonal lattice structure with the lattice constant of 14 to 15 nm when suspended in 50 mm tris(hydroxymethyl)aminomethane buffer at pH 8.5 containing 10 mm Mg 2+ . Under this experimental condition, effects of other divalent metal cations on the hexagonal assembly of the electrodialyzed LPS were compared with that of Mg 2+ . The Zn 2+ , Hg 2+ , Cu 2+ , and Ni 2+ could produce essentially the same hexagonal lattice structure with the lattice constant of 14.5 to 15.0 nm as that formed with Mg 2+ . The Cd 2+ , Co 2+ , and Fe 2+ produced the hexagonal lattice structure with the lattice constant of 15.5 to 16.0 nm, and Ba 2+ , Sr 2+ , and Ca 2+ produced that with the lattice constant of 18 to 19 nm. In addition, the hexagonal lattice structures formed with the latter three cations were less orderly than those formed with the other cations. When the higher concentrations of Ba 2+ , Sr 2+ , and Ca 2+ were used, the lattice constants were not shortened. The length of lattice constants of the hexagonal lattice structures formed with the divalent cations did not relate to the quantity of the cations bound to the LPS. Among the divalent cations tested, Hg 2+ was bound to the LPS in the smallest amount (its atomic ratio to P, 0.07), and Zn 2+ and Fe 2+ were bound in very large amounts (their atomic ratios to P, 2.94 and 8.28, respectively).