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Regulation of in vivo Immunological Reactions by Monoclonal Antibody against Lymphocyte Activation Antigen
Author(s) -
Yuasa Hiroo,
Uede Toshimitsu,
Wada Takuro,
Yamaguchi Akira,
Murakami Toshiya,
Osawa Hisao,
Diamantstein Tibor,
Kikuchi Kokichi
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01399.x
Subject(s) - monoclonal antibody , biology , in vivo , antigen , immunology , lymphocyte , antibody , lymphocyte activation , t lymphocyte , immune system , t cell , genetics
In vivo effects of a monoclonal antibody that recognizes rat lymphocyte activation antigen were studied. Spleen cells obtained from sheep red blood cell (SRBC)‐immunized rats developed strong PFC response against SRBC. However, the 5C6‐F4 treatment resulted in the inhibition of subsequent development of PFC response. The suppression of PFC response was due to the inhibition of generation of helper T cells, but not due to the preferential induction of suppressor cells. In addition, 5C6‐F4 antibody was also found to inhibit the clinical expression of collagen‐induced rat arthritis and the synovial inflammation in collagen‐induced arthritis rats. Furthermore, the in vivo generation of cytotoxic cells against syngeneic tumor cells was also inhibited by 5C6‐F4 antibody. The in vivo administration of 5C6‐F4 antibody did not cause any pathological changes in brain, lung, liver, kidney, spleen, thymus, and lymph nodes.