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Chemotactic Response of Human Neutrophils to N ‐Acetyl Chitohexaose in vitro
Author(s) -
Tokoro Akio,
Suzuki Ko,
Matsumoto Tatsuji,
Mikami Takeshi,
Suzuki Shigeo,
Suzuki Masuko
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01398.x
Subject(s) - chemotaxis , phospholipase a2 , cyclooxygenase , in vitro , biology , neutrophile , lipoxygenase , eicosanoid , n formylmethionine leucyl phenylalanine , stimulation , prostaglandin , pharmacology , immunology , biochemistry , enzyme , endocrinology , receptor , arachidonic acid
N ‐Acetyl chitohexaose (NACOS‐6) was able to display chemotactic response of human neutrophils in vitro. In order to analyze the mechanism, a series of chemotaxis studies by means of neutrophils treated with inhibitors of phospholipase A 2 , cyclooxygenase, or lipoxygenase to NACOS‐6 was conducted. The treatment of neutrophils with inhibitors of phospholipase A 2 or cyclooxygenase resulted in decrease of number of migrated cells. However, the lipoxygenase inhibitors did not exhibit the same effect. On the other hand, the treatment of neutrophils with inhibitors of phospholipase A 2 or lipoxygenase resulted in decrease of chemotactic response to Formyl‐Met‐Leu‐Phe (FMLP), although the cyclooxygenase inhibitors did not inhibit chemotaxis of neutrophils. Neutrophils added to exogenous prostaglandin E 2 (PGE 2 ) caused an enhanced chemotactic response to NACOS‐6. These results indicate that the mechanism of chemotactic response to NACOS‐6 was different from that of FMLP, and that the response was enhanced by PGE 2 released from the neutrophils with stimulation of NACOS‐6.