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The Structure of Marek's Disease Virus DNA: Amplification of Repeat Sequence in IR s and TR s
Author(s) -
Hayashi Masanobu,
Jessip John,
Fukuchi Kunihiko,
Smith Mary,
Tanaka Akiko,
oyama Meihan
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01386.x
Subject(s) - biology , southern blot , dna , marek's disease , dna sequencing , microbiology and biotechnology , virus , genome , repeated sequence , nucleic acid sequence , sequence analysis , virology , genetics , gene duplication , long terminal repeat , sequence (biology) , inverted repeat , gene
The structure of Marek's disease virus (MDV) DNA was investigated by using Southern blot hybridization analysis. A heterogeneous region was observed in the inverted repeats region, IR s and TR s , as well as in the TR L and IR L . The results of DNA sequencing of the heterogeneous region showed that the heterogeneity of IR s and TR s was due to amplification of a 178‐bp repeat sequence. Amplification of IR s and TR s was found in viral DNA from both pathogenic and nonpathogenic strains. The structure of DNA from the latent MDV genome present in established lymphoblastic cells was also determined. Amplification of the 132‐bp repeat sequence in IR L and TR L was not found in latent MDV DNA of established lymphoblastic cells, whereas amplification of the 178‐bp repeat sequence in IR s and TR s was found in the same DNA.