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Further Evidence for Heterogeneity of Fcγ‐Receptors on Guinea Pig Splenic B and T Lymphocytes: Analysis Using Monoclonal Antibodies to Two Distinct Types of Fcγ‐Receptor
Author(s) -
Yamashita Toshiyuki,
Koyama Jiro
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01378.x
Subject(s) - antibody , biology , microbiology and biotechnology , receptor , monoclonal antibody , fc receptor , guinea pig , fragment crystallizable region , immunology , biochemistry , endocrinology
In our previous paper, we reported that guinea pig splenic lymphocytes expressed two distinct Fc‐receptors for homologous IgG (FcγRs), one monospecific for IgG2 (Fcγ 2 R) and the other bispecific for IgG1 and IgG2 (Fcγ 1 /γ 2 R), when analyzed by EA‐rosette assay. These FcγRs on the cells were further studied by using two monoclonal antibodies toward the FcγRs on guinea pig peritoneal macrophages (anti‐Fcγ 1 /γ 2 R and anti‐Fcγ 2 R antibody). The anti‐Fcγ 1 /γ 2 R antibody completely inhibited the rosette formation of splenic lymphocytes with IgG1‐sensitized sheep erythrocytes [EA(IgG1)]. On the other hand, EA(IgG2)‐rosette formation was inhibited partially by anti‐Fcγ 2 R but not by anti‐Fcγ 1 /γ 2 R antibody. Complete inhibition of the EA (IgG2)‐rosette formation was achieved by simultaneous additions of both anti‐Fcγ 2 R and anti‐Fcγ 1 /γ 2 R antibodies. The binding of IgG2 antibody complexed with ovalbumin to the cells was partially inhibited by either anti‐FcγR antibody, and complete inhibition occurred in the presence of both the antibodies, indicating that two types of FcγR, Fcγ 1 /γ 2 R, and Fcγ 2 R, are expressed on the cells. The determination of these FcγRs on B and T lymphocytes by two‐color flow cytometry showed that about 52% of B lymphocytes expressed Fcγ 1 /γ 2 R alone and 32% of the cells expressed both the FcγRs. On the other hand, about 12% of T lymphocytes was found to express Fcγ 2 R alone and the cells expressing Fcγ 1 /γ 2 R were in the minority (3.8%). T lymphocytes expressing both the FcγRs were not detected. These results show that guinea pig B lymphocytes bear two types of FcγRs and are heterogeneous with regard to their FcγRs and that T lymphocytes express Fcγ 2 R mainly.