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Characterization of Rat T Cell Subset Antigen by Monoclonal Antibody
Author(s) -
Yamaki Toshiaki,
Uede Toshimitsu,
Sugawara Yukihiro,
Wada Takuro,
Yamaguchi Akira,
Kokai Yasuo,
Kikuchi Kokichi
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03141.x
Subject(s) - antigen , biology , microbiology and biotechnology , epitope , mixed lymphocyte reaction , clone (java method) , spleen , t lymphocyte , monoclonal antibody , t cell , lymph node , antigen presenting cell , lymphocyte , cytotoxic t cell , antibody , immune system , immunology , in vitro , biochemistry , dna
6B2‐B8 T cell hybridoma cells were used to immunize mice, and immune spleen cells were fused with NS/1 myeloma cells. One clone, designated RTH‐7, reacted with 89.5% of rat thymocytes, 30.2% of rat spleen cells, and 42.3% of rat lymph node cells. The RTH‐7 reacted with a subset of rat T cells but not with B cells. Double staining analysis demonstrated that RTH‐7 stained a rat T cell subset distinct from R1‐10B5‐positive cells that were known to be equivalent to mouse Lyt‐2. It was revealed that RTH‐7 and W3/25 recognize different antigenic epitopes on the same molecule. The RTH‐7 as well as W3/25 substantially inhibited the production of interleukin 2 by cells in mixed lymphocyte reaction and the lymphocyte proliferation induced by mixed lymphocyte reaction. The RTH‐7 inhibited the lymphocyte proliferation induced by Con A whereas W3/25 failed to do so. The RTH‐7 defined antigen has a molecular weight of 53,000 under reducing condition and 47,000 under nonreducing condition. The RTH‐7 defined antigen showed a wide range of heterogeneity in pI (6.2–8.8). The associated molecule of approximate molecular weight of 27,000 was occasionally detected with the RTH‐7 defined antigen in 6B2‐B8 T cell hybridoma cells as well as peripheral T cells but not in thymocytes. Thus, RTH‐7 detects a cell surface antigen of a functional T cell subset of rat origin.