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Quantitative Evaluation of Colonizing Ability of Vibrio cholerae O1
Author(s) -
Nakasone Noboru,
Iwanaga Masaaki
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03137.x
Subject(s) - vibrio cholerae , biology , microbiology and biotechnology , vibrionaceae , cholera , pathogenicity , vibrio , bacteria , genetics
A new method to evaluate the adhesive ability of Vibrio cholerae O1 was proposed. Broth cultured V. cholerae O1 and a piece of formalin‐fixed rabbit intestinal wall were incubated together in KRT buffer and the number of adhered organisms was counted under a scanning electron microscope. This method was much less laborious than other methods that have been used so far, and most significantly, constant results were obtained in repeated experiments. The adhesive properties of toxigenic V. cholerae O1 evaluated by this method correlated well with its observed experimental pathogenicity.