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Chemical Properties of Lipopolysaccharide‐Like Substance (LLS) Extracted from Leptospira interrogans Serovar canicola Strain Moulton
Author(s) -
Shimizu Tadayori,
Matsusaka Eiichiro,
Nagakura Naoki,
Takayanagi Kazuhisa,
Masuzawa Toshiyuki,
Iwamoto Yoshihisa,
Morita Tamotsu,
Mifuchi Ichiji,
Yanagihara Yasutake
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03133.x
Subject(s) - leptospira interrogans , rhamnose , xylose , chemistry , biochemistry , chromatography , fucose , mannose , fatty acid , galactose , leptospira , microbiology and biotechnology , biology , fermentation , serotype
The aqueous layer was isolated from Leptospira interrogans serovar canicola strain Moulton by the hot phenol‐water method. After ultracentrifugation, the precipitate was designated as lipopolysaccharide‐like substance (LLS) fraction and the chemical composition was compared with that of bacterial LPS. The LLS fraction consists of 35.2% carbohydrate, 3.8% amino sugar, 36.4% lipid, 15.2% protein, and 0.3% phosphorus. Neutral sugars were detected as rhamnose, arabinose, xylose, 4‐ O ‐methylmannose, mannose, galactose, and a small amount of erythrose, fucose and glucose by gas‐liquid chromatography (GLC), but 2‐keto‐3‐deoxyoctonic acid was not detected in the LLS by thiobarbituric acid test and high voltage paper electrophoresis. Fatty acids detected by GLC were decanoic acid (C10: 0), dodecanoic acid (C12: 0), dodecenoic acid (C12: 1), tridecenoic acid (C13: 1), tetradecanoic acid (C14: 0), hexadecanoic acid (C16: 0), hexadecenoic acid (C16: 1), and octadecenoic acid (C18: 1). With SDS‐polyacrylamide gel electrophoresis, bacterial LPS showed many orderly bands, while the banding pattern of the leptospiral LLS was very simple. These findings demonstrate that the physicochemical properties and chemical composition of LLS fraction from Leptospira are different from those of LPS extracted from gram‐negative bacteria such as Enterobacteriaceae , and suggesting that Leptospira has no typical LPS.