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Application of a New Medium Supplement for Propagation and Storage of Human Cytomegalovirus
Author(s) -
Okada Masanobu,
Minamishima Yoichi
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03126.x
Subject(s) - human cytomegalovirus , fetal bovine serum , biology , growth medium , virology , in vitro , virus , biochemistry , bacteria , genetics
A new medium supplement, NU‐SERUM, was evaluated for cultivation of human embryonic lung fibloblasts (HEL) and for propagation and storage of human cytomegalovirus (HCMV). NU‐SERUM was comparable to fetal bovine serum (FBS) in promoting rapid growth of HEL if they were seeded at a sufficient density. HCMV replicated quite satisfactorily in HEL cultured with media supplemented with NU‐SERUM as well as FBS. Inactivation of HCMV at 37 C occurred similarly when the medium contained FBS or NU‐SERUM. However, at —70 C, HCMV was less stable in NU‐SERUM‐containing medium than in FBS‐containing medium. Sorbitol added to the NU‐SERUM‐containing medium improved the unstableness of HCMV at —70 C, and HCMV was storable with such medium. Thus, NU‐SERUM is useful as an alternative to FBS not only for growth of HEL but also for propagation and storage of HCMV.