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Demonstration of Cross‐Reactive Antibodies to Mycoplasmas in Human Sera by ELISA and Immunoblotting
Author(s) -
Sasaki Tuguo,
Bonissol Christiane,
Stoiljkovic Bob,
Ito Kenichiro
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03124.x
Subject(s) - mycoplasma pneumoniae , mollicutes , mycoplasmataceae , antibody , biology , titer , mycoplasma , microbiology and biotechnology , cross reactivity , blot , virology , immunology , cross reactions , medicine , pneumonia , biochemistry , gene
Varying levels of cross‐reactivity to some mycoplasma species were observed in the sera of patients infected with Mycoplasma pneumoniae and even in normal human sera by enzyme‐linked immunosorbent assay (ELISA). The absorption of the patients' sera with M. pneumoniae lysate showed the decrease in ELISA titers not only to M. pneumoniae , but also to other mycoplasma species. These results suggested the existence of cross‐reactive antibodies to mycoplasmas in human sera. Cross‐reactive antibodies to M. pneumoniae and other mycoplasmas in the patients' sera were also demonstrated by Western blotting technique.