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Isolation of Campylobacter pyloridis from Human Gastric Mucosa and Characterization of the Isolates
Author(s) -
Itoh Takeshi,
Yanagawa Yoshitoki,
Shingaki Masao,
Takahashi Masaki,
Kai Akemi,
Ohashi Makoto,
Hamana Gennichi
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03121.x
Subject(s) - nalidixic acid , campylobacter , biology , gastric mucosa , gastroenterology , gastritis , microbiology and biotechnology , urease , medicine , gastric polyp , stomach , bacteria , tetracycline , biochemistry , antibiotics , enzyme , genetics
Biopsy specimens of human gastric mucosa of patients with gastric complaints and subjected to endoscopic examination were cultured microaerobically, and Campylobacter pyloridis was detected in 46 out of 80 cases (57.5%). The organism was found in 13 out of 22 patients with gastritis, 11 out of 16 with gastric ulcer scar, 7 out of 16 with gastric ulcer, 3 out of 9 with gastric polyp, 4 out of 5 with gastric carcinoma, 2 out of 2 with esophagus carcinoma, and 6 out of 9 with other gastric diseases. The isolates were identified as C. pyloridis , demonstrating its characteristic features such as positive for oxidase and catalase, negative for reduction of nitrite and nitrate, positive for urease, no growth at 25 C, growth at 37 C, not tolerant to 1% glycine, and resistant to nalidixic acid. Positive alkaline phosphatase activity was considered as an additional feature characteristic for the strains of C. pyloridis . The major cellular fatty acids were tetradecanoic acid and 19‐carbon‐cyclopropane acid. This pattern is unique among Campylobacter species. The survival of the organism for a longer period than 60 min at pH 2.5 indicates its significant resistance to acidic environment.