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Human Macrophage‐Activating Factors for Cytotoxicity
Author(s) -
Higuchi Masahiro,
Sugimoto Masamichi,
Kobayashi Yoshiro,
Osawa Toshiaki
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03110.x
Subject(s) - macrophage activating factor , cytotoxicity , lymphokine , biology , size exclusion chromatography , priming (agriculture) , microbiology and biotechnology , molecular mass , recombinant dna , lipopolysaccharide , biochemistry , in vitro , immunology , botany , germination , gene , enzyme
Two different factors (MAF‐C I and MAF‐C II) were obtained by anion exchange chromatography of the culture supernatant of a human T‐cell hybridoma, H3‐E9–6, which produces macrophage‐activating factors for cytotoxicity (MAF‐C). These 2 factors induced the cytotoxicity of monocytes synergistically as a priming signal (MAF‐C I) and a triggering signal (MAF‐C II), respectively. On gel filtration on a column of Superose 12, MAF‐C II was eluted mainly at the void volume, whereas MAF‐C I was eluted in the fractions corresponding to approximate molecular weights of 30–300 K. On the other hand, gel filtration in the presence of sodium deoxycholate revealed that MAF‐C II has an approximate molecular weight of 40,000, but MAF‐C I was unstable under these conditions. When the activity for mouse macrophages (MAF‐Cm activity) was tested, the MAF‐C II fraction showed high MAF‐Cm activity in the presence of murine recombinant interferon gamma (rIFN‐ γ ), but the MAF‐C I fraction did not show MAF‐Cm activity even in the presence of lipopolysaccharide (LPS). These results suggest that MAF‐C I (priming lymphokine) has species specificity but MAF‐C II (triggering lymphokine) does not.