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Immunogenetic Analysis of Tolerance Induction in Anti‐Alloantigen Delayed Type Hypersensitivity Responses by Portal Venous Pre‐Inoculation with Allogeneic Cells
Author(s) -
Shimizu Jun,
Ogata Masato,
Qian JiaHua,
Sato Sohichiro,
Kokudo Shuhei,
Hamaoka Toshiyuki,
Fujiwara Hiromi
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03097.x
Subject(s) - spleen , immunology , immune tolerance , biology , antigen , major histocompatibility complex , mhc class ii , immune system , immunization , splenocyte , delayed hypersensitivity
The present study investigates some of the immunogenetic bases for tolerance of anti‐allo‐delayed type hypersensitivity (DTH) responses as induced by pre‐inoculating allogeneic cells via portal venous (p.v.) route. BALB/c mice were injected with totally allogeneic C57BL/6 or H‐2 incompatible BALB.B spleen cells via p.v. route. These mice not only failed to exhibit anti‐H‐2 b DTH responses, but also abrogated the potential to generate H‐2 b ‐specific DTH responses as induced by the subsequent immunization with H‐2 b spleen cells via subcutaneous (s.c.) route. The p.v. presensitization with allogeneic spleen cells differing at either class I or class II of major histocompatibility complex (MHC) resulted in the tolerance induction of DTH responses to the respective allogeneic class I or class II MHC antigens. Moreover, the p.v. administration of the class I‐positive allogeneic cell fraction depleted of class II‐positive component into recipients differing at both class I and class II was capable of inducing anti‐class I DTH tolerance. These results indicate that anti‐allo‐class I or class II DTH tolerance can be induced independently and that the existence of class II antigens on p.v.‐presensitized cells is not necessarily required for the tolerance induction of anti‐allo‐class I DTH response.