Premium
Interleukin 1 Production and Accessory Cell Function of Rat Alveolar Macrophages Exposed to Mineral Dust Particles
Author(s) -
Oghiso Yoichi,
Kubota Yoshihisa
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03090.x
Subject(s) - concanavalin a , alveolar macrophage , in vitro , pulmonary alveolus , biology , macrophage , interleukin 2 , microbiology and biotechnology , immunology , chemistry , biochemistry
Immunostimulatory effects of respirable mineral dust particles on alveolar macrophages (AM) and T lymphocytes were tested in vitro . When rat AM were incubated with fibrogenic silica and asbestos particles, a significant interleukin 1 (IL‐1) activity was generated into the culture supernatants, whereas neither AM alone nor AM incubated with non‐fibrogenic titanium dioxide (TiO 2 ) particles, however, produced a detectable amount of IL‐1. Interleukin 2 (IL‐2) activity, as tested with IL‐2‐dependent CTLL‐2 assays, was not detectable from all of these cultures. It was also revealed that concanavalin A‐induced proliferation of T lymphocytes was enhanced in autologous AM and nylon wool‐passed spleen cell co‐cultures incubated only with fibrogenic particles, but not with non‐fibrogenic particles. Higher IL‐1 activity was detected only from co‐cultures exposed to fibrogenic particles, whereas IL‐2 activity was almost similar among these co‐cultures. These results indicate the differences in immunostimulatory effects on pulmonary macrophages and T lymphocytes among a variety of mineral dust particles.