Premium
Total Nucleotide Sequences of the Infectious Cloned DNAs of Bean Golden Mosaic Virus
Author(s) -
Morinaga Tsuto,
Ikegami Masato,
Shimotohno Kunitada,
Miura KinIchiro
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03078.x
Subject(s) - orfs , biology , nucleic acid sequence , nucleotide , dna , genetics , open reading frame , dna sequencing , homology (biology) , rna , virology , microbiology and biotechnology , gene , peptide sequence
Complete nucleotide sequences of the infectious cloned DNA components (DNA A and B) of bean bolden mosaic virus were determined. The DNA A (2585 nucleotides) and DNA B (2647 nucleotides) have little sequence homology with each other, but both A and B contain a common region of 205 nucleotides. A possible large hairpin structure is detected in the common region. Nucleotide sequences of DNAs A and B revealed the presence of 8 potential coding regions for proteins (m.w.> 10,000). Among them, four open reading frames (ORFs 1–4) encode proteins of m.w. 30,000 or greater, and are individually coded in virion DNA A and B senses (+) and their complementary senses (‐), respectively. The other four ORFs 5–8 are in virion DNA B(+) and its complementary sense (‐). All of the ORFs 1–4 have regulatory signals for RNA synthesis (TATAA/T) in the region 5′ upstream from a potential start codon ATG.