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Effect of Cytolytic Infection on Maintenance of Resistance to HVJ (Sendai Virus) in an Altered BHK Cell Culture
Author(s) -
Yokoo Junko,
Miyadai Toshiaki,
Yokochi Takashi,
Kimura Yoshinobu
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03077.x
Subject(s) - baby hamster kidney cell , biology , sendai virus , infectivity , virology , virus , cell culture , cytolysis , reversion , colcemid , cytotoxicity , in vitro , genetics , gene , phenotype
Altered baby hamster kidney (BHK‐R) cells were serially cultured in the continuous presence of hemagglutinating virus of Japan (HVJ). These cells showed a distinct resistance to superinfection with the homologous HVJ. This resistance of BHK‐R cells gradually disappeared after serial passages in the presence of ultraviolet‐irradiated HVJ particles which lost infectivity but still preserved hemagglutinating and neuraminidase activities. When BHK‐R cells were serially cultured in the presence of a temperature‐sensitive mutant of HVJ at non‐permissive temperature, the cells also lost the resistance. The resistance of BHK‐R cells remained unchanged, even after prolonged incubation in virus‐free maintenance medium under the conditions of no cell division. It was suggested that killing of virus‐sensitive cells, which were generated during cell proliferation, was required for maintenance of the resistance.