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Detection of Cross‐Reactive Antibody to BLV p24 in Sera of Human Patients Infected with HTLV
Author(s) -
Onuma Misao,
Tsukiyama Kyoko,
Ohya KenIchi,
Morishima Yasuo,
Ohno Ryuzo
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb03076.x
Subject(s) - bovine leukemia virus , antigen , antibody , virology , biology , monoclonal antibody , immunofluorescence , leukemia , microbiology and biotechnology , immunodiffusion , virus , immunology
For detection of antibody to bovine leukemia virus (BLV) major core protein of p24 and cross‐reactive antibody in human patients infected with human T cell leukemia virus type I (HTLV‐I), monoclonal antibody, D432 against BLV p24 was used by competitive binding enzyme‐linked immunoadsorbed assay (ELISA). In sera from cattle with enzootic bovine leukosis (EBL) which were positive for BLV antibodies by immunodiffusion test, 109 out of 112 (97.3%) were positive for BLV p24 antibody by competitive binding ELISA. By using the same procedures, 21 samples from adult T cell leukemia (ATL) patients and healthy carriers with HTLV‐I were tested for cross‐reactive antibody to BLV p24. All 21 samples were positive for HTLV‐I antibodies by immunofluorescence test and/or ELISA. By competitive binding ELISA using non‐treated BLV antigens, none of these 21 samples inhibited the binding of the D432. When the BLV antigen was treated by several different denaturation procedures, several HTLV‐I positive samples showed the inhibition of the D432 binding and the most effective treatment was by 2‐mercaptoethanol (2‐ME). Sixteen out of 21 samples showed the presence of cross‐reactive antibody against 2‐ME‐treated BLV antigens. The cross‐reactivity of human sample to BLV p24 antigen was further confirmed by Western blotting of the 2‐ME‐treated BLV antigens. None of the 28 samples from leukemia patients other than ATL which were negative for HTLV‐I antibodies showed inhibition of the D432 by the competitive binding ELISA.