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Augmentation of Killer Activity of Murine Spleen Cells against Allogeneic and Syngeneic Tumor Cells by Inoculation of Alloantigen in vivo
Author(s) -
Nishi Yasuaki,
Hosokawa Tomohide,
Aoike Akira,
Kamahora Takeshi,
Sudo Hiromasa,
Kawai Keiichi
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb01357.x
Subject(s) - cytotoxic t cell , mastocytoma , spleen , lymphokine activated killer cell , biology , antigen , cytotoxicity , immunology , in vivo , in vitro , microbiology and biotechnology , interleukin 12 , biochemistry
After C57BL/6 (B6) mice were inoculated with BALB/c spleen cells via tail vein, kinetics of cytotoxic activities in the B6 mice against sensitizing alloantigens (H‐2 d ) and against syngeneic antigens were investigated using, as target cells, P815 mastocytoma cells (H‐2 d ) and B16 melanoma cells (H‐2 b ). Cytotoxic activity against P815 in the B6 spleen cells reached a peak 3 days after alloantigen inoculation, decreased drastically on day 5 and rose again thereafter. The profile of anti‐B16 cytotoxic activity was similar to that of anti‐P815 activity. The cytotoxic activity against P815 was inhibited partially by cold B16, but that against B16 was not inhibited by cold P815. Surface phenotype of cytotoxic cells against P815 was Lyt 2+ , Thyl + , Asialo GM1 + and that of cytotoxic cells against B16 was Lyt2 − , Thyl +/− , and Asialo GMl + . The results indicate that inoculation of B6 mice with allogeneic BALB/c spleen cells induce two types of cytotoxic cells; one is similar to lymphokine‐activated killer (LAK) cells and the other is activated natural killer cells.