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Effect of Histamine and Antihistamines on Interleukin‐1 Production by Human Monocytes
Author(s) -
Manosroi Jiradej,
Manosroi Aranya,
Vithayasai Vicharn
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb01355.x
Subject(s) - histamine , cimetidine , antagonist , hydrochloride , lipopolysaccharide , endocrinology , pharmacology , medicine , receptor antagonist , biology , chemistry , receptor , biochemistry
This study was carried out on the effect of histamine hydrochloride and its antagonists on the production of interleukin‐1 (IL‐1) by lipopolysaccharide (LPS)‐stimulated adherent human monocytes (AHM) from normal healthy blood donors. IL‐1 activity was evaluated by incorporation of [ 3 H]‐thymidine in mouse thymocytes in samples of 1:3 dilution. The result indicated that histamine hydrochloride significantly suppressed IL‐1 production by AHM at 10 −3 m and 10 −10 m in 14 donors with maximal suppression observed at 10 −3 m. A 1‐hr incubation with histamine hydrochloride (10 −3 m) before addition of LPS was found to be appropriate. Cimetidine, an H 2 ‐antagonist at 10 −3 m, 10 −5 m, and 10 −7 m significantly inhibited the effect of histamine hydrochloride (10 −3 m) and gave maximum inhibition at 10 −5 m, whereas chlorpheniramine maleate, and H 1 ‐antagonist had no significant inhibitory effect at the concentrations studied (10 −4 m, 10 −5 m, and 10 −7 m). Histamine hydrochloride (10 −3 m) added alone had no significant suppressive effect, while cimetidine (10 −5 m) alone had a significant stimulatory effect on IL‐1 production by AHM.