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Protection against Campylobacter jejuni Infection in Suckling Mice by Anti‐Flagellar Antibody
Author(s) -
Ueki Yuji,
Umeda Akiko,
Fujimoto Shuji,
Mitsuyama Masao,
Amako Kazunobu
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb01350.x
Subject(s) - polyclonal antibodies , flagellin , biology , antibody , campylobacter jejuni , microbiology and biotechnology , monoclonal antibody , immunoelectron microscopy , immunoglobulin a , immunoglobulin g , bacteria , virology , immunology , genetics
We obtained two monoclonal antibodies of IgM class and IgA class of immunoglobulin prepared from mouse spleen cells immunized with crude flagellar preparation, and a polyclonal antibody raised against purified flagellin monomer of Campylobacter jejuni in a rabbit. The specificity of the reaction of these antibodies for flagellar filament was confirmed by Western blotting and by immunoelectron microscopy. These antibodies caused agglutination of the bacteria and inhibited the motility of the bacteria. When a strain of C. jejuni was treated with IgM class monoclonal antibody before being inoculated into suckling mice, it reduced colonization of the intestinal tract by this bacteria. Inhibition of the colonization by IgA class monoclonal antibody was less effective than that of IgM class, and the polyclonal antibody consisting mostly of IgG class immunoglobulin was without effect.