Premium
The Role of Interleukin 1 and 2 in Generation of Acquired Resistance against Mouse Typhoid Infection Afforded by Dialyzable Factor from Salmonella typhimurium
Author(s) -
Kita Eiji,
Emoto Masashi,
Nishi Kazuyuki,
Katsui Noriaki,
Kashiba Shuzo
Publication year - 1987
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1987.tb01336.x
Subject(s) - splenocyte , salmonella , microbiology and biotechnology , biology , listeria monocytogenes , in vitro , lymphokine , concanavalin a , antigen , interleukin , immunology , cytokine , bacteria , biochemistry , genetics
Dialyzable factor (DF) prepared from a ribosomal fraction of Salmonella typhimurium was tested for its ability to induce interleukin 1 (IL 1) and 2 (IL 2) production, in relation to acquired resistance, after an intraperitoneal injection of DF. IL 1 production in vitro by peritoneal macrophages of DF‐treated mice reached the maximum 4 days after injection, at the time when the nonspecific local resistance via macrophages directly activated with DF became apparent (Kita et al, Microbiol. Immunol. 28 : 807, 1984). Concanavalin A‐induced IL 2 production by splenocytes of DF‐treated mice reached the maximal level between days 6 and 8, and it could be enhanced even on day 14. Antigen‐induced blastogenic responses of splenocytes from DF‐treated mice reached the maximal level 14 days after treatment. Although DF did not show the mitogenic activity to normal splenocytes, T cells of DF‐treated mice could respond to S. typhimurium. On the contrary, T cells of normal mice could respond to heat‐killed cells of S. typhimurium when they were cultured with macrophages which had been directly stimulated in vitro with DF. Furthermore, T cells from DF‐treated mice could respond to antigens of different species of bacteria, and especially to Listeria monocytogenes. These results suggest that T cells of DF‐treated mice, being at the intermediate stage of activation via monokines including IL 1 which is produced by macrophages stimulated with DF, are able to proliferate immediately after the administration of challenging organisms as a second signal, and also that the specificity of the response may be defined by the challenging organisms.