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Rabbit Autoantibodies to Actin Induced by Immunization with Modified Homologous Actins
Author(s) -
Nishioka Mikio,
Aibiki Toshiyuki,
Shirai Mutsunori,
Terada Soichiro,
Kagawa Hiroyuki,
Watanabe Seishiro
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03061.x
Subject(s) - homologous chromosome , autoantibody , actin , heterologous , biology , antibody , sodium dodecyl sulfate , microbiology and biotechnology , skeletal muscle , virology , immunology , biochemistry , anatomy , gene
Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits, thus immunized, produced antibodies reactive with actins of homologous and heterologous animals. In addition, the antibodies showed reactivity with autologous actin. It indicates that the denatured homologous actin is capable of terminating immunological tolerance to actin and induces formation of autoantibody to rabbit actin. This phenomenon may be implicated in the occurrence of anti‐actin antibody in sera from patients with chronic liver disease and several other diseases.