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Enhanced Interleukin 1 Production by Alveolar Macrophages and Increase in Ia‐Positive Lung Cells in Silica‐Exposed Rats
Author(s) -
Oghiso Yoichi,
Kubota Yoshihisa
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03047.x
Subject(s) - lipopolysaccharide , bronchoalveolar lavage , biology , lung , immunology , inhalation , pulmonary alveolus , cytokine , interleukin 2 , microbiology and biotechnology , andrology , medicine , respiratory disease , anatomy
Inhalation exposure to silica dust enhanced interleukin 1 (IL‐1) production by alveolar macrophages (AM), which is attributable to an increase in Ia‐positive lung cells. While the proportion of Ia‐positive cells in lavaged bronchoalveolar cells (BAC) was much lower (0–3%) in unexposed control rats, about a third of the rats that inhaled silica showed higher proportions (8.0–18.5%); these were designated “Ia‐high” exposed animals. The number of total cells, Ia‐positive cells and lymphocytes in BAC was significantly increased ( P <0.05, P <0.001, and P <0.001, respectively) in these “Ia‐high” exposed animals, compared to the control animals. Adherent AM populations obtained from BAC preparations also contained significantly higher ( P <0.001) proportions of Ia‐positive cells in the “Ia‐high” exposed animals. When these adherent AM cultures were stimulated with lipopolysaccharide, IL‐1 activity of the culture supernatants was enhanced and was significantly higher ( P <0.001) in the “Ia‐high” exposed rats, compared to the control animals. These results indicate that silica‐exposure can induce populational changes in lung cells and also activation of AM associated with the increase in Ia‐positive cells.