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A Monoclonal Antibody with Broad Reactivity to Human Interferon‐α Subtypes Useful for Purification of Leukocyte‐Derived Interferon
Author(s) -
Tsukui Kazuo,
Uchida Shigeharu,
Tokunaga Eiichi,
Kawade Yoshimi
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03042.x
Subject(s) - alpha interferon , polyclonal antibodies , interferon , monoclonal antibody , biology , microbiology and biotechnology , antibody , sendai virus , recombinant dna , interferon alfa , virology , polyacrylamide gel electrophoresis , monoclonal , virus , biochemistry , immunology , enzyme , gene
A monoclonal antibody to human interferon‐α, termed HT‐1 antibody, with a broad reactivity to various subtypes of interferon‐α was prepared. It bound and neutralized all of the four subtypes of E. coli ‐derived human recombinant interferon‐α (aL 1 , aL 2 , aL 4 , and aL 6 ) tested; it also neutralized human natural leukocyte interferon but only partially. Human interferon‐β and ‐γ were not bound. The antibody conjugated to Sepharose beads retained over 90% of human leukocyte interferon induced by Sendai virus. The bound interferon was recovered by acid elution in good yields and in almost pure form (specific activity was about 2 ′ 10 8 international units/mg protein). The purified interferon showed, in SDS‐polyacrylamide gel electrophoresis, an activity profile with major peaks in a mol. wt. range of 17,000–22,000, which completely agreed with the profile shown by polyclonal antibody‐purified interferon. Such purified leukocyte interferon‐α preparations containing most of the naturally occurring subtypes can be useful for clinical and other purposes.