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In Vitro Hexagonal Assembly of Lipopolysaccharide of Escherichia coli K‐12
Author(s) -
Kato Nobuo,
Ohta Michio,
Kido Nobuo,
Ito Hideo,
Naito Setsuko,
Kuno Tsuneharu
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03040.x
Subject(s) - magnesium , hexagonal crystal system , calcium , lattice constant , lipopolysaccharide , escherichia coli , crystallography , sodium dodecyl sulfate , salt (chemistry) , sodium , crystal structure , hexagonal lattice , ethanol , biology , biophysics , biochemistry , chemistry , organic chemistry , diffraction , immunology , physics , antiferromagnetism , gene , optics , condensed matter physics
We examined Escherichia coli K‐12 lipopolysaccharide (LPS), which is known to be an R‐form LPS, for its ability to form a hexagonal lattice structure in vitro . The LPS from E. coli K‐12 strain JE1011 did not form a hexagonal lattice structure when it was precipitated by addition of two volumes of 10 mm MgCl 2 ‐ethanol, but it did form such a structure when it was electrodialyzed and then converted to the magnesium or calcium salt form. The lattice constant of the magnesium salt form was 15.2±0.3 nm and that of the calcium salt form 18.5±0.3 nm. Since prior treatment of the LPS with proteinase K in the presence of sodium dodecyl sulfate did not affect its capability of hexagonal assembly, the lattice formation by the LPS does not require the presence of proteins.