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Culture Conditions for Stimulating Cholera Toxin Production by Vibrio cholerae O1 El Tor
Author(s) -
Iwanaga Masaaki,
Yamamoto Koichiro,
Higa Naomi,
Ichinose Yoshio,
Nakasone Noboru,
Tanabe Masao
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03037.x
Subject(s) - vibrio cholerae , el tor , cholera toxin , latex fixation test , toxin , microbiology and biotechnology , enterotoxin , yeast extract , biology , cholera , vibrionaceae , vibrio , food science , bacteria , escherichia coli , fermentation , antibody , immunology , biochemistry , genetics , gene
A method that stimulates cholera toxin (CT) production by Vibrio cholerae O1 biotype El Tor (El Tor vibrios) to the level of several micrograms per ml in the culture fluid was established. Such a large amount of CT was obtained by the following method: El Tor vibrios were cultured in AKI medium (1.5% Bacto peptone, 0.4% yeast extract‐Difco, 0.5% NaCl, 0.3% NaHCO 3 ) at 37 C for 4 hr in a stationary test tube and then for 16 hr in a shaken flask, with inoculum sizes of 10 5 to 10 7 /ml. With this method, 35 strains out of 60 examined produced 2 to 16 μ g/ml of CT as determined by the reversed passive latex agglutination test (RPLA). Thirty‐three randomly selected strains out of the 60 produced reasonable amounts of rabbit skin vascular permeability factor, reflecting the amount of CT titrated with RPLA.