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Human Interferon‐ γ (IFN‐ γ ) Containing Cells Bear Various Surface Phenotypic Markers
Author(s) -
Sugawara Isamu,
Kitagawa Hiroshi,
Inagaki Hirofumi,
De Ley Mark,
Fukuda Akio
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03034.x
Subject(s) - biology , flow cytometry , phenotype , population , cell , interferon , microbiology and biotechnology , receptor , cell culture , immunology , biochemistry , gene , genetics , demography , sociology
We explored the population of Interferon‐ γ (IFN‐ γ ) containing cells in order to clarify their cell surface phenotypic markers. Here we define γ ‐IFN containing cells as γ ‐IFN plaque forming cells (PFC). By this method, it was found that IFN‐ γ containing cells consist of two cell fractions, i.e ., OKT3 + , OKT4 + , and OKT8 ‐ cells and OKM1 + cells. Effective IFN‐ γ production seems to require participation of plastic‐adherent cells (presumably monocytes), while the addition of cyclosporin A (CyA) almost completely blocked generation of human IFN‐ γ . To characterize Con A‐stimulated IFN‐ γ containing cells, we performed two‐color flow cytometry using FACS IV. Most of the IFN‐ γ containing cells have surface phenotypic markers for Leu 3 , Leu 8 , Leu15, HLA‐DR, and IL‐2 receptors, but most lack markers for Leu2 and Leu7. Interestingly, most of Leu3 + and IL‐2 receptor + cells belong to the dimly illuminating cell fractions of the IFN‐ γ containing cell population. Our results indicate that IFN‐ γ containing cells are heterogeneous with respect to surface phenotypic markers but the predominant IFN‐ γ containing cell type is the helper T cell (OKT4 + ). Lastly, OK432, glycyrrhizin, and CCA (lobenzarit disodium) increase the number of IFN‐ γ containing cells and are thought to be immunomodulators.