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Effect of Mannitol and Glucose on the Distribution and Trypsin Susceptibility of Protein A of Staphylococcus aureus
Author(s) -
Yamada Sakuo,
Matsumoto Akira
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03029.x
Subject(s) - mannitol , staphylococcus aureus , biology , trypsin , biochemistry , protein a , protein biosynthesis , strain (injury) , ferritin , microbiology and biotechnology , enzyme , bacteria , immunology , genetics , anatomy , antibody
The biological activity and morphological distribution of protein A on the cell surface were studied in a medium containing an excess of either mannitol or glucose, which suppressed protein A production of Staphylococcus aureus , Cowan I strain. Preculture of the organisms in the presence of a sugar suppressed the expression of protein A, resulting in a decrease in the number of cells bound with anti‐ferritin rabbit IgG molecules, which specifically indicate protein A distribution. The distribution pattern of protein A on the cell surface changed with glucose but not with mannitol. The two‐layered ferritin distribution on the organism grown in the control medium was altered into a heavily labeled, thick and rough layer with glucose, suggesting the induction of a conformational change in the polypeptide chain forming protein A. This was positively supported by the increase in trypsin susceptibility of protein A.