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Stimulation of Macrophages and Antitumor Activity of Radiodetoxified Endotoxin
Author(s) -
Nerkar Devdas Punjaji,
Bandekar Jayant Ramchandra
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03016.x
Subject(s) - lipopolysaccharide , stimulation , salmonella , biology , ratón , microbiology and biotechnology , pharmacology , macrophage , bacteria , immunology , biochemistry , endocrinology , in vitro , genetics
Lipopolysaccharide of Salmonella typhimurium was irradiated with gamma radiation at 10, 15, and 30 kGy doses. A dose of 30 kGy significantly detoxified the LPS (180 times). Mice were injected intraperitoneally with the radiodetoxified LPS, and it was found that it stimulated peritoneal macrophages as was evident from the enhancement of their acid hydrolases and cellular RNA content. Both LPS and radiodetoxified LPS exhibited antitumor activity against S180 cells in Swiss mice. Treatment with 20 μg/mouse of either LPS or 30 kGy LPS gave maximum survival of the mice (90%). These mice were found to resist the challenge of S180 cells (1 × 10 6 ).