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Lipid Metabolism in Endotoxic Rats
Author(s) -
Kawakami Masanobu,
Murase Toshio,
Itakura Hiroshige,
Yamada Nobuhiro,
Ohsawa Nakaaki,
Takaku Fumimaro
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03012.x
Subject(s) - lipoprotein lipase , triglyceride , very low density lipoprotein , endocrinology , lipase , biology , medicine , enzyme , hepatic lipase , cholesterol , lipoprotein , lipid metabolism , metabolism , biochemistry
Studies were conducted to investigate the effect of E. coli endotoxin administration on hepatic triglyceride lipase (H‐TGL) activity in rats, since H‐TGL activity is known to behave differently from lipoprotein lipase (LPL) activity in various situations. Plasma triglyceride and free fatty acid concentrations were markedly elevated in animals after injection of endotoxin. Cholesterol and phospholipids were also increased significantly. Lipoprotein analysis by ultracentrifugation showed that the most pronounced increase of lipoproteins was in the VLDL and IDL fractions. Triglyceride lipase activities in post‐heparin plasma were markedly decreased. A selective assay for H‐TGL activity using a specific antibody revealed that this enzyme as well as LPL is significantly decreased (26% of control) in endotoxic animals. Thus, the increase of VLDL and IDL appears to result from the decrease of both of LPL and H‐TGL.