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Role of L3T4 Antigen in the Activation of Various Functions of Lyt‐1 + 2 ‐ T Cells against Vaccinia Virus
Author(s) -
Aoyama Atsuo,
Yoshioka Takayuki,
Sato Soichiro,
Mizushima Yumiko,
Ogata Masato,
Ueda Shigeharu,
Kato Shiro,
Fujiwara Hiromi,
Hamaoka Toshiyuki
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03006.x
Subject(s) - vaccinia , lymphokine , biology , antigen , cytotoxic t cell , virus , virology , t cell , t lymphocyte , interleukin 2 , immune system , microbiology and biotechnology , immunology , in vitro , recombinant dna , biochemistry , gene
The present study defines assay systems for vaccinia virus‐reactive Lyt‐1 + 2 ‐ T cells mediating various functions and investigates the positivity of L3T4 antigen on these Lyt‐1 + 2 ‐ T cells as well as the role of L3T4 antigen in the activation of these T cells with respect to their functions. C3H/He mice were immunized against vaccinia virus by inoculating viable virus intraperitoneally (i.p.). Antivaccinia virus reactivity in lymphoid cells from these immunized mice was assessed by a) proliferative response, b) helper T cell activities involved in cytotoxic T lymphocyte (CTL) and B cell (antibody) responses, c) delayed type‐hypersensitivity (DTH) response, and d) production of lymphokines such as interleukin 2 (IL2) and macrophage‐activating factor (MAF). The results demonstrate that all of the above anti‐vaccinia virus responses were mediated by Lyt‐1 + 2 ‐ T cells and that these Lyt‐1 + 2 ‐ T cells expressed L3T4 antigens on their cell surfaces. Moreover, such anti‐vaccinia Lyt‐1 + 2 ‐ T cell responses were inhibited in the presence of anti‐L3T4 antigen antibody. These results indicate that there is a reciprocal relationship between Lyt‐2 and L3T4 markers, and that L3T4 antigen is closely related to the activation of various functions of anti‐vaccinia virus Lyt‐1 + 2 ‐ T cells.