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Staining of O‐Specific Polysaccharide Chains of Lipopolysaccharides with Ruthenium Red
Author(s) -
Kuno Tsuneharu,
Naito Setsuko,
Ohta Michio,
Kido Nobuo,
Ito Hideo,
Kato Nobuo
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb03001.x
Subject(s) - ruthenium red , staining , strain (injury) , polysaccharide , ruthenium , biology , microbiology and biotechnology , klebsiella , negative stain , bacteria , salmonella , biochemistry , chemistry , escherichia coli , anatomy , gene , genetics , electron microscope , physics , organic chemistry , optics , calcium , catalysis
S‐form lipopolysaccharides (LPS) from Klebsiella strain LEN‐1 (O3: K1 —) and from Salmonella minnesota strain 1114 were positively stained with ruthenium red, whereas R‐form LPS from Klebsiella strain LEN‐111 (O3— : K1 —) and Ra, Rb 1 , RcP + , Rd 1 P ‐ , and Re LPS from the respective mutant strains of S. minnesota were not or only faintly stained by such treatment. From these results it was concluded that ruthenium red stains the O‐specific polysaccharide chains of LPS. The appearance of stained preparations of S‐form LPS suggested that the material responsible for this positive staining corresponded to the surface projections which were seen by the negative staining technique as attached to the ribbon‐like structures and spherules of the LPS.