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Serological Differences between Human T‐lymphotropic Virus Type‐I (HTLV‐I) and HTLV‐III/LAV
Author(s) -
Harada Shinji,
Purtilo David T.,
Koyanagi Yoshio,
Yamamoto Naoki
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb02979.x
Subject(s) - virology , serology , titer , antibody , immunofluorescence , virus , biology , leukemia , human t lymphotropic virus , t cell leukemia , human t lymphotropic virus 1 , neutralizing antibody , immunology , neuroscience , myelopathy , spinal cord
Sera from each of five preselected groups of patients with acquired immune deficiency syndrome (AIDS), AIDS‐related complex (ARC), hemophilia, adult T‐cell leukemia (ATL), and healthy controls were examined for antibodies to human T‐cell leukemia (T‐lymphotropic) virus type‐I (HTLV‐I) and HTLV‐III by indirect immunofluorescence (IF) and radioimmunoprecipitation (RIP) methods. All sera from five patients with AIDS, ARC, and hemophilia reacted at titers from 1: 512 to 1: 5,120 with fixed H9/HTLV‐III cells by IF but not with fixed MT‐1 cells carrying HTLV‐I. Similarly, sera from patients with AIDS, ARC, and hemophilia precipitated HTLV‐III‐specific polypeptides of 120K, 46K, and 24K. In contrast, sera from five patients with ATL did not react with fixed H9/HTLV‐III cells, but reacted with fixed MT‐1 cells. Moreover, HTLV‐I‐specific polypeptides of 68K, 28K, and 24K were precipitated with sera from ATL‐patients but not with anti‐HTLV‐III‐positive sera. Recently, we infected HTLV‐I‐carrying MT‐4 cells with HTLV‐III and provoked strong cytopathic effects. This system enabled testing for neutralizing antibodies to HTLV‐III. Neutralizing titers to HTLV‐III of five anti‐HTLV‐III‐positive sera ranged from 1:720 to 1:9,000. In contrast, all five seronegative controls showed no or only low reactivity to HTLV‐III envelope (1:80 and 100). However, three out of five anti‐HTLV‐I‐positive sera exhibited weak cross‐reactivities with HTLV‐III. The reactivities were expressed as <1:160, except for one case (1:720). They were considered to be nonspecific since they were negative for HTLV‐III antibodies in the radioimmunoprecipitation studies.