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A Probable New Adhesive Factor (F42) Produced by Enterotoxigenic Escherichia coli Isolated from Pigs
Author(s) -
Yano Tomomasa,
Leite Domingos Da Silva,
De Camargo Irineu José Barsanti,
De Castro Antonio Fernando Pestana
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb02976.x
Subject(s) - fimbria , microbiology and biotechnology , enterotoxigenic escherichia coli , biology , enterotoxin , bacterial adhesin , antiserum , escherichia coli , antigen , biochemistry , gene , genetics
Three enterotoxigenic Escherichia coli (ETEC) strains (coded 62, 104, and 567/7) isolated from piglets with neonatal diarrhea produced only a thermostable enterotoxin. Although these strains showed mannose‐resistant microhemagglutination (MRMH), the responsible factor was serologically different from the known hemagglutinating colonization factors from porcine strains (K88, K99, and F41). Bacterial cells from these strains adhered to HeLa cells and pig brush borders. Electron microscope studies revealed the presence of fimbria‐like structures on bacterial cells grown at 37 C but not on cells grown at 18 C. The antiserum prepared from partially purified fimbrial antigen (provisionally called F42) inhibited chicken erythrocyte MRMH caused by these strains as well as adherence of strain 567/7 to HeLa cells and to pig brush borders. These data taken together suggest the existence of a new hemagglutinating adhesin that is different from those so far described for porcine ETEC.