Premium
Number of Hits Necessary for Complement‐Mediated Hemolysis
Author(s) -
Takeda Junji,
Kozono Haruo,
Takata Yuko,
Hong Kyongsu,
Kinoshita Taroh,
Sayama Koji,
Tanaka Etsuko,
Inoue Kozo
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb02972.x
Subject(s) - hemolysis , lysis , complement (music) , immune system , complement system , biology , immunology , microbiology and biotechnology , biochemistry , phenotype , gene , complementation
The number of hits necessary for the C8 and C9 steps of immune hemolysis was reexamined with a previously unemployed experimental design, in which various numbers of EAC1–7, excess of the supplementary component and a constant amount of the component tested were incubated in a constant volume (Inoue et al. 1976. Infect. Immun. 13 : 337). Our results were consistent with previous findings; the steps of guinea pig C8 and C9, and human C8 each followed a one‐hit mechanism, while that of human C9 showed a multi‐hit response. When lysis of sensitized erythrocytes (EA) by normal human serum was analysed in a similar way, one‐hit curves were obtained. This result, taken together with the above results, suggests that immune hemolysis occurs by a single lesion including a single C8 and multiple C9 in the case of human complement and that normal human serum contains sufficient excess of C9. On the other hand, when C9‐deficient human serum was used for lysis of EA, multiple‐hit curves were obtained. The mechanism of lysis by C5b‐8 may differ from that by C5b‐9.