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Properties of Monospecific Antibodies to the Glycoprotein of Western Equine Encephalitis Virus
Author(s) -
Yamamoto Kiichi
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb00951.x
Subject(s) - neutralization , virology , biology , antiserum , antibody , epitope , monoclonal antibody , glycoprotein , virus , hemagglutination assay , population , hemagglutination , microbiology and biotechnology , immunology , titer , medicine , environmental health
Monospecific (MSp‐) antisera against E1 and E2 glycoproteins of western equine encephalitis (WEE) virus were prepared and examined for binding activities to whole virions, hemagglutination‐inhibition (HI), neutralization (NT) and protection. Both anti‐E1 and anti‐E2 MSp‐antibodies (Abs) showed HI activity, but not NT activity. Both NT‐negative MSp‐Abs protected mice against WEE virus challenge. A competition experiment with monoclonal antibodies showed that these MSp‐antisera appear to lack the antibody population for some epitopes involved in viral neutralization.