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Immunochemical Similarity of Synthetic α‐(1→3)‐Branched D ‐Glucans to Dextran B1375
Author(s) -
Torii Mitsuo,
Yamazoe Mari,
Ogawa Setsuko,
Watabe Kazuhito,
Koshikawa Tomihiko,
Uryu Toshiyuki,
Schuerch Conrad
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb00941.x
Subject(s) - dextran , leuconostoc mesenteroides , antiserum , biology , antibody , biochemistry , branching (polymer chemistry) , chemistry , bacteria , immunology , organic chemistry , lactic acid , genetics
Anti‐dextran B1375 antibodies were raised in rabbits by injecting formalin‐killed Leuconostoc mesenteroides strain NRRL B1375, and the anti‐dextran serum was used to examine native dextran B1375, and synthetic linear and four α‐(1→3)‐branched α‐(1→6)‐d‐glucopyranans for similarities. The antiserum reacted with the homologous dextran B1375 and also with all the synthetic linear and branched glucans. Precipitation and precipitation‐inhibition studies indicated that the antiserum contained at least three groups of antibodies with different specificities, the first specific for linear α‐(1→6)‐D‐glucopyranan structure, the second specific for α‐D‐glycopyranosyl‐(1→3)‐branching and the last specific for another, unknown structure present in the dextran B1375 molecule. Two samples of the synthetic branched glucans were shown to be immunochemically the most similar to natural dextran B1375 by inhibition experiments.