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Antigen Presentation by Human Antigen‐Presenting Cells to Antigen‐Specific Xenogeneic Murine T Cells
Author(s) -
Yabu Kouji,
Yano Akihiko
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb00939.x
Subject(s) - antigen , biology , antigen presenting cell , antigen presentation , microbiology and biotechnology , keyhole limpet hemocyanin , t cell , cytotoxic t cell , clone (java method) , immune system , immunology , in vitro , biochemistry , dna , genetics
Successful antigen presentation by xenogeneic human antigen‐presenting cells (APC) to stimulate the proliferation of antigen‐specific, keyhole limpet hemocyanin (KLH)‐specific, ovelbumin (OVA)‐specific, and purified protein derivative of Mycobacterium tuberculosis (PPD)‐specific murine T cells was observed. Evidence indicating a direct cell interaction between antigen‐specific murine T cells and xenogeneic human APC was given by experiments using antigen‐specific murine T cell clones. The OVA‐specific B10.S(9R) T cell line (9‐0‐A1) and PPD‐specific B10.A(4R) T cell line (4‐P‐1) were stimulated by both xenogeneic human APC and murine APC from syngeneic or I‐A compatible strains, while the PPD‐specific human T cell line (Y‐P‐5) was stimulated by autologous human APC but not by murine APC. Anti‐HLA‐DR monoclonal antibodies (MoAb) blocked the xenogeneic human APC‐antigen‐specific murine T cell clone interaction. Thus, human xenogeneic APC can stimulate antigen‐specific murine T cells through HLA‐DR molecules in the same manner as syngeneic murine APC do through Ia molecules coded for by the I region of the H‐2 complex, while murine APC failed to present antigen to stimulate human antigen‐specific T cells.