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The Production of a Cytotoxic Factor by Mouse Peritoneal Macrophages and Macrophage Hybridomas Treated with Various Stimulating Agents
Author(s) -
Takeda Yasuhisa,
Higuchi Masahiro,
Sugimoto Masamichi,
Shimoda Olina,
Woo Hee Jong,
Shimada Shigetoshi,
Osawa Toshiaki
Publication year - 1986
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1986.tb00929.x
Subject(s) - lipopolysaccharide , macrophage , cytotoxic t cell , macrophage activating factor , stimulation , biology , immunology , microbiology and biotechnology , cytotoxicity , macrophage colony stimulating factor , lymphokine , immune system , in vitro , endocrinology , biochemistry
Murine peritoneal macrophages elicited with a streptococcal preparation, OK‐432, produced as much of a cytotoxic factor after stimulation with lipopolysaccharide (LPS) as BCG‐elicited macrophages did. Proteose peptone‐elicited macrophages produced a very small amount, if any, of the factor, and resident peritoneal macrophages did not release it at all even after LPS‐stimulation. A newly established macrophage hybridoma, D/O‐3.3, produced the factor after LPS‐stimulation, but another hybridoma, D/O‐3.2, did not. Experiments using these peritoneal macrophages and macrophage hybridomas demonstrated that macrophages can be divided into three subpopulations with regard to stages of activation for production of the cytotoxic factor. The first is fully activated macrophages which produce the factor after stimulation with LPS or MAF‐C alone, the second is partially activated macrophages which produce the factor only after stimulation with a combination of recombinant interferon‐γ (rlFN‐γ) and LPS or rIFN‐γ and macrophage activating factor for cytotoxicity (MAF‐C), and the third is nonactivated macrophages which cannot produce the factor at all.