Premium
Formation of a Hexagonal Lattice Structure by an R‐Form Lipopolysaccharide of Klebsiella : Comparative Study of Preparations Extracted by the Phenol‐Water and the Phenol‐Chloroform‐Petroleum Ether Methods
Author(s) -
Kato Nobuo,
Ohta Michio,
Kido Nobuo,
Ito Hideo,
Naito Setsuko,
Kuno Tsuneharu
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00895.x
Subject(s) - chloroform , phenol , petroleum ether , solvent , ether , chromatography , chemistry , hexagonal crystal system , nuclear chemistry , crystallography , extraction (chemistry) , organic chemistry
Abstract An R‐form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN‐111 (O3‐:K1‐) by the phenol‐chloroform‐petroleum ether method was compared with that extracted by the phenol‐water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol‐water method and dialyzed against tap water to remove phenol showed ribbon‐like structures, and it formed a hexagonal lattice structure with a lattice constant of 14.5 ± 0.3 nm when it was precipitated by addition of two volumes of 10 m M MgCl 2 ‐ethanol. The LPS which was extracted by the phenol‐chloroform‐petroleum ether method and lyophilized consisted of ribbon‐like structures and their fragments and it often formed small pieces of a hexagonal lattice, although the LPS before lyophilization did not form such a lattice. When the LPS extracted by the phenol‐chloroform‐petroleum ether method was precipitated by addition of two volumes of 10 m M MgCl 2 ‐ethanol, it formed essentially the same hexagonal lattice structure as that formed by the LPS extracted by the phenol‐water method. From these results it is concluded that the ability of the LPS to form a hexagonal lattice structure does not depend upon the method of its extraction from bacterial cells.