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Persistence of Mumps Virus in Mouse L929 Cells
Author(s) -
Ito Yasuhiko,
Tsurudome Masato,
Hishiyama Michiko
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00887.x
Subject(s) - vero cell , virology , biology , cell culture , mumps virus , pi , virus , microbiology and biotechnology , biochemistry , genetics
The characteristics of a persistent infection of L929 cells with mumps virus (MuV) is presented. The persistent infection (L‐MuV cells) was regulated by interferon (IFN) produced endogenously and almost all the properties showed that the carrier culture was maintained by horizontal transmission of the virus. Small‐plaque mutants, but not temperature‐sensitive variants, were selected during the persistent infection. MuV released from L ‐MuV cells (MuV‐pi) replicated efficiently in L929 cells, while infection of L929 cells with the original MuV‐o resulted in an abortive infection. The efficient replication of MuV‐pi in L929 cells can be explained by the findings that MuV‐pi induced IFN more slowly and had lower susceptibility to IFN in L929 cells than MuV‐o did. M protein was synthesized to a considerable degree in MuV‐pi‐infected cells, while it could not be detected in MuV‐o‐infected cells. By contrast, MuV‐pi formed small plaques in Vero cell monolayers and the yield of MuV‐pi in Vero cells was lower than that of MuV‐o. M protein induced by MuV‐pi decayed easily in Vero cells. M protein was considered to be a limiting factor for MuV replication in both cell lines.