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Relationship between β‐Lactamase Activity and Resistance to β‐Lactam Antibiotics in Mycobacterium smegmatis
Author(s) -
Yabu Kunihiko,
Kaneda Shunko,
Oghiai Toshiro
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00883.x
Subject(s) - microbiology and biotechnology , penicillin , mycobacterium smegmatis , mutant , biology , clavulanic acid , cephalosporin , antibiotics , mutagenesis , cefoxitin , bacteria , amoxicillin , mycobacterium tuberculosis , genetics , gene , staphylococcus aureus , medicine , tuberculosis , pathology
Penicillin‐susceptible mutants and β‐lactamase‐negative mutants were isolated from Mycobacterium smegmatis after nitrosoguanidine mutagenesis. Both the mutants were found to be susceptible to low levels of penicillin and cephalosporins by twofold dilution testing. Clavulanic acid reduced the minimal inhibitory concentrations of β‐lactamase‐labile β‐lactams for the penicillin‐susceptible mutants and the parent strain, but had no effect on the susceptibility of the β‐lactamase‐negative mutants. Comparison of the β‐lactamase activities found in these mutants and the parent strain indicated that there was a rough correlation between the β‐lactamase level in these organisms and their susceptibility to β‐lactams.