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Anti‐O‐Phosphotyrosine Antibodies in Human Sera
Author(s) -
Ogawa Ryoichi,
Ohtsuka Masahiro,
Noguchi Kenichi,
Iwamura Kenichiro,
Watanabe Yasushi
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00879.x
Subject(s) - biology , antibody , virology , immunology , microbiology and biotechnology
Antibodies reactive with O ‐phosphotyrosine (PTYR) were detected in 60 out of 621 inpatients, with high frequencies in hematologic and lung malignancies, hepatic diseases, cerebrovascular diseases, and autoimmune diseases. Affinity‐purified antibodies proved capable of recognizing PTYR‐containing proteins in a human carcinoma cell line, A431, both by immunofluorescent staining and by immunoaffinity chromatography, but had no detectable affinity for phosphorylated serine or threonine, or for the nucleotides tested. In these respects, the antibodies observed in human sera were indistinguishable from anti‐PTYR antibodies raised experimentally in rabbits or mice.