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Possible Involvement of Transglutaminase in Endocytosis and Antigen Presentation
Author(s) -
Teshigawara Keisuke,
Kannagi Reiji,
Noro Nobuhiro,
Masuda Tohru
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00877.x
Subject(s) - antigen , biology , antigen presentation , internalization , receptor , endocytosis , microbiology and biotechnology , tissue transglutaminase , immune system , pinocytosis , t cell , immunology , biochemistry , enzyme
Experiments were carried out to determine as to whether or not internalization of antigen is necessary for subsequent antigen presentation by accessory cells using monoamines which are known as transglutaminase (TGase) inhibitors. It was found that endoeytosis for immune complexes via Fc receptors such as sheep erythrocytes coated with IgG class antibody (EA) was different from receptor‐independent endoeytosis for soluble protein such as horse radish peroxidase (HRP) in the sensitivity to monoamines; methylamine inhibited the receptor‐dependent endoeytosis of immune complexes at a concentration of over 20 m M and the receptor‐independent endoeytosis of HRP at 2 m M , while dansylcadaverine (DC) inhibited both at a concentration of 100 μ M . It was noteworthy that antigen‐specific T cell proliferation to splenic adherent cells pulsed with DNP 9,6 ‐ovalbumin (DNP 9,6 ‐OVA) was blocked strongly by DC as well, but weakly by methylamine. These results suggest the possibility that antigen presentation requires internalization of antigen by a mechanism such as receptor‐dependent endoeytosis for the subsequent reexpression of antigen on membranes. Furthermore, it was confirmed that TGase activity is high in peritoneal exeudate and spleen adherent cells, both of which have accessory cell activities for lymphocytes, suggesting the possibility that TGase might be involved intimately in receptor‐dependent endoeytosis and subsequent antigen presentation.