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Formation of Varicella‐Zoster Virus Antigens in Infected Vero Cells
Author(s) -
Takayama Michiko,
Oya Akira
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00867.x
Subject(s) - virology , vero cell , biology , varicella zoster virus , antigen , virus , immunology
The formation of varicella‐zoster (V‐Z) virus‐associated antigens was studied in V‐Z virus‐infected Vero cells by means of indirect immunofluorescence. Early antigen (EA) was first detected inside V‐Z virus‐infected Vero cells 4 to 6 hr after infection, whereas surface membrane antigen (SMA) was expressed on the outer surface of infected cells 2 to 3 hr later than EA, and intranuclear late antigen (LA) was detected several hours later than SMA antigen. EA expression was not inhibited by cytosine arabinoside (Ara‐C) treatment, whereas LA formation was completely blocked by Ara‐C. The presence of two components of SMA early SMA (ESMA) and late SMA (LSMA), was suggested by this difference in susceptibility to Ara‐C. The formation of all viral antigens, EA, SMA, and LA, was blocked by inhibitors of RNA and protein synthesis.